Cloning of gene AsTP1 transactivated by arsenic trioxide

  Objective   To clone new gene AsTP1 transactivated by arsenic trioxide.

  Methods   Suppression subtractive hypridization(SSH)technique was used.the mRNA was isolated from Jurkat cells treated with arsenic trioxide(5 mol/L) and 0.9 percent sodium chloride, respectively.then cDNA was synthesized.SSH method was employed to analyze the differ entially expressed DNA sequence between the two groups.On the base of subtractive cDNA library of genes transactivated by arsenic trioxide, the coding sequence of new gene was obtained by bioinformatics methods.the reverse transcription PCR (RT-PCR)was used to amplify the new gene from the mRNA of Jurkat cells and HepG2 cells; this new gene named as AsTP1.the sequece for the AsTP1 gene was deposited into GenBank, and the accession number was AY605064.

  Results   the coding sequence of new gene was cloned and identified successfully.

  Conclusion   A gene is recognized as the new target gene transactivated by arsenic trioxide.the results will pave the way for the study of the molecular mechanism of the transactivating effects of arsenic trioxide and the development of new clue for carcinogenic mechanism of chronic arsenisim.