Objective To establish a specific and sensitive serological method,ELISA,for detection of rubella virus(RV) antibody using yeast expression products as coating antigen and to improve its sensitivity and specificity.
Methods The yeast expression products and RV culture medium were used as coating antigens respectively to assay 90 sera.The same sera were also assayed by JINGMEI kit and Germany RECI kit.The results of Germany kit,as a golden standard,were compared with those of the other three methods in specificity,sensitivity and accordance rate.And the statistical test was used to compare the differences among these methods,and also to evaluate which was more useful for coating antigen,recombinant antigen or RV culture medium.
Results The results of repeated experiment of recombinant protein showed that there was no statistical significance between two experiments(P>0.05).The difference of the two positive rates between RECI kit and recombinant antigen was significant(P<0.05).The difference of the two positive rates between RECI kit and RV culture medium was significant(P<0.05).There was statistical significance between those of recombinant antigen and RV culture medium(P<0.05).
Conclusion As coating antigen,the recombinant E1 Protein is better than that of RV culture medium in EL ISA method for detection of RV antibody.
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