Effect of protein kinase C on hippocampal neurons apoptosis induced by hyperthermia

  Objective   To investigate the effect of protein kinase C on the hippocampus neurons apoptosis induced by hy perthermia and to provide the evidence for the prevention of brain injuries caused by hyperthermia.

  Methods   By establishing the apoptosis model of primary culture of hippocampal neurons induced by hy perthermia, the dynamic change of concentration of Ca²⁺ in hippocampal neurons and the influence to Bcl-2 expression were observed after using chelerythr ine chloride(CTC) which was the special inhibitor of PK C.

  Results   The concentration of Ca²⁺ in hippo campal neurons increased rapidly with hyperthermia treatment(42)for 1 h and application of CTC.The climax of increase was arrived after 25~30 s, and de creased slowly until to keep the higher level than that of normal level.The expression of Bcl-2 in hippocampal neurons with hyperthermia and CTC treatment decreased dist inctly more than that of cells in 42 for 1 h.

  Conclusion   The activation of PKC played a very important protection role in the apoptosis induced by hyperthermia in hippocampal neurons.The application of CTC influenced greatly the concentration of Ca²⁺ and the expression of Bcl-2 in hippocampal neurons after hyperthermia.