ELISA reagent for detecting hemorrhagic fever with renal syndrome by using antibody of Hantaan virus

  Objective   To detect Hemorr hagic Fever with Renal Syndrome(HFRS)earlier and sensitively, the recombinant nucleocapsid protein(rNP)and monoclonal antibody(McAb)of Hantaan virus were used and ELISA reagent was allocated.

  Methods   The genome S 1.3 kb of Hantaan virus 76 118 was transfered to plasmid of E.coli to express nucleo capsid protein.Lymphocytes B were taken from mice which were immunized by the rNP.The sensitized lymphocytes Band myeloma cells were fused together for getting the McAb of rNP, and IgM capturing ELISA was established with rNP and McAb.Our ELISA reagent was compared with IFAand ELISA reagent which was produced with American rNP and McAb.

  Results   The sensitivity and specificity of our ELISA reagent met the standar d of the ELISA reagent with American rNP and McAb completely.

  Conclusion   It is great meaningful for using our IgM capturing ELISA to detect HFRS.