Objective To study the dynamic effects of N-Methyl-N-Nitrosoguanidine(MN NG)on carbohy drate in the cultured cells from adult Schistosoma japanicum.
Methods The cultur ed cells from 32-day old S.japonicum were inoculated on cover slips and cultured in routine medium which was made up of RPMI-1640, 20% calf serum and moderate amount of antibiotics.At the 4th day, the cultured cells were div ided into test group and control group at random.The cells in the test group were treated with MNNG which final concentration in the routine medium was 3 μg/ml for 48 h, whereas those in the control group were treated with the routine medium free MNNG for the same time.After being washed tho roughly, they went on being cultured in the routine medium for 3 weeks; then were cultured in the low-serum medium which serum concentration was just 5%.From the 1st to 8th week, the cells were stained weekly to show the content and distribution of hepatin and carbo hydrate by Periodic A cid Scid Schiff reaction(PAS)and PAS staining after the cells were digested with amylase.The contents of carbohydrate in stained cells at the 6th week(the 5th week after inducement)were measured by using photometerimage analy tical instrument(HPIAS-2000)and analy zed by statistic method.
Results Along with the extending of the culture time, the staining of the cultured cells in the control group became weak, whereas those in the test group became strong.The contents of hepatin and carbohy drate of the cell in the test grop were significantly higher than those in the control group(P < 0.01).A the 6th week, not only the staining of the cells treated by MNNG were the deepest, but also the 2nd type cells and dividing cells incr eased significantly.
Conclusion MNNG could increase evidently the dividing cells and the content of hepatin and carbohydr ate in the cultured cells from adult S.japonicum.
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