Distribution and characterization of Shiga toxin-producing E.coli from different samlpes

  Objective   To explore the distribution and character ization of Shig a tox in-producing E.coli from animal feces, meat food and contaminatemud.

  Methods   Routine method was used to isolate E.coli, strains of Shiga tox in1-2 producing were detected by PCR assay; and types were analyzed by ser ological technique.

  Results   Of all samples, 8 isolates were identified as Shiga toxin-producing E.coli, one strain was identified as EHEC O157:H7, two strains as EHEC O157:H7 with no Shiga tox in and five strains as no-EHEC O157:H7 with producing Shiga toxin.

  Conclusion   ST ECcould be separated from different samples, and there was slightly distance between types and pathogenic factors.