Combinative toxicity of Microcystin, Aflatoxin and Fumonisin

  Objective   To study 50% inhibition concentration(IC₅₀)of Microcystin, Aflatoxin and Fumonisin and the combinative toxic effect of the three biotoxins in Hep G2 cell line.

  Methods   Cell viability and 50% inhibition concentration (IC₅₀)of Microcystin-L R(MCLR), Aflatoxin B₁(AFB₁), Fumonisin B₁(FB₁)and the mixture were measured by tetrazolium salt(WST-1)assay with Hep G2 cell line.Combinative toxicity of the three biotoxins was evaluated according to response surface analysis.

  Results   Treatment of MCLR for 24, the IC₅₀ was more than 100μmol/L.IC₅₀ of Aflatoxin B₁, Fumonisin B₁ and the mixture of the three biotoxins were 1.0, 399.2 and 172.0μmol/L respectively.The interaction index according to response surface analysis was 0195.

  Conclusion   Combinative toxicity of the mixture falls into the category of additive effect.