Effect of soybean isoflavonet on bone morphogenetic protein 2 expression of cultured osteoblasts from rat

  Objective   To study the effect of soybean isoflavone on bone morphogenetic protein 2(BMP ₂)expression of cultured osteoblasts from rat.

  Methods   The osteoblstic cells from cranial bone of newborn rat was cultured.Cultured rat osteoblasts were treated with soybean isoflavone in difierent concentrations.Total RNA was extracted from the cells.BMP ₂ were amplified by RT-PCR.β-actin cDNA was also amplified at the same time and used as an internal standard.The SP method was used to detect the effect of soybean isoflavone on BMP ₂ expression of cultured osteoblasts.The expression level was monitored with HPIAS-2000 photograph analysis meter.The pictures of PCR production was scanned.The specific values of density of BMP ₂/β-actin were calculated, and the expression levels of BMP ₂ were determined.

  Results   Soybean isoflavone increased the level of BMP ₂ in rat osteoblasts and increased the expressions of BMP ₂ genes at the level of transcripaon and revealed dose-effect relation(P < 0.01).

  Conclusion   The soybean isoflavone induces the proliferation and differentiaion of osteoblast probably by increasing the expressions of BMP ₂ genes.