Effect of bisphenol-A on apoptosis of male mice reproductive cells

  Objective   To study the effects of bisphenol-A(BPA)on apoptosis of male mice reproductive cells.

  Methods   Male mice in experiment groups were exposed to BPA by using the intraperitoneal injection for 5d at the dose of 0, 250, 500, 1000μmol/kg respectively.Seven mice were randomly sacrificed in each group on the 77th and 14th day after first exposure to BPA.The flow cytometry was used to study the effects of BPA on testicle cells of male mice.The testicular viscera coefficient were measured and the contents of nitric oxide(NO)and nitric oxide synthase(NOS)were tested.

  Results   The different dose of BPA could make the testicle cell cycle change.The cells in G₀/G₁, Sphases decreased, the accounts of cells in G₂/M increased and Ap part increased with the increase of BPA.The testicular viscera coefficient decreased in the experimental groups compared with that in the blank group.The contents of NO and NOS in the testicles of mice were higher than that in the blank group.There were significant differences between experimental groups and blank control.

  Conclusion   BPA can inhibit DNA synthesis of testicle cells, cause the G₂ block and delay the mitosis of testice cells, and the cells in Mphase decreased with the increase of BPA.It shows that BPA may affect the reproductive cells on apoptosis of male mice.The content of NO and NOS increased to show that the damaged reproductive cells are related with lipid peroxide.