Scavenging effect of water-soluble chitosan on hydroxyl radical

  Objective   To detect the hydroxyl radical-scavengingeffect of water-soluble chitosan by phenanthroline-Fe(Ⅱ)oxidation assay.

  Methods   In Fenton reaction system, ascorbic acid(VC)was used as positive control, and the hydroxyl radical-scavengingeffect of water-soluble chitosan with different concentration was tested.

  Results   (1) It showed that the highest rate of hydroxyl radical-scavengingof water-soluble chitosan could be 93.67%;(2)The pseudo-positive scavengingeffect on hydroxyl radical of water-soluble chitosan with the highest value of 9.03% was tested specially in the experiment.The rate of hydroxyl radical-scavengingof the chitosan was still up to 74.98% ~84.64% when the rate of pseudo-positive scavenging was calibrated.

  Conclusion   Water-soluble chitosan is an appropriate kind of hydroxyl radical-scavenging agent.