Protection of polyphenols of vitis amurensis rupron on injured vascular endothelial cells in vitro

  Objective   To evaluate the effects of the polyphenols of Vitis amurensis Rupron injured vascularendothelium cells.

  Methods   The experiment were performed in culture of H₂O₂-induced human umbilical vein endothelium cell(ECV) in vitro.Cell viability was assessed by MT Tassay.Radioimmunoassay was used to assess the released level of endothelin-1(ET-1)and prostacyclin(PGI₂).

  Results   H₂O₂ inhibited the ECVproliferation.Pre-incubation of ECVwith the polyphenols of Vitis amurensis Ruprbefore H₂O₂ exposure significantly increased the cell viability, the polyphenols of Vitis amurensis Ruprreduced LDH, ET-1 and increased the level of PGI₂ in H₂O₂-induced ECV.

  Conclusion   The po lyphenols of Vitis amurensis Ruprcan produce the protective action on injured ECV.