Epidemiology survey on virological diarrhea among children in rural of Qinhuangdao city

  Objective   To investigate the epidemiological characterstus of rotanvirus and caliciviruses among children under 5 years of age with acute diarrhea.

  Methods   Two viruses were detected by enzyme linked immunosorbent assay ELISAand reverse transcription-polymerase chain reaction(RT-PCR), some caliciviruses were cloned and sequenced.

  Results   1 236 fecal specimens were detected for rotavirus, 46% was positive and 393 rotavirus negative which fecal specimens were detected for caliciviruses 31.6% was positive.The distribution of month-old The highest was 12 month group for rate of Two virus were infected.followed rotavirus was 9 month group(26.1%)6 month group(14.4%)and calicivirus was 6 month group(25.6%), 9~month group(17.9%).The distribution of seasons two riruses maily were infected in 12 month, 1month and 2 month.Rotavirus Gserotyping results identified G₃(45%)to be the most common strain.followed by G₁ (35%), G₂(8%), G₄(3%), G₉(0.6%).mixed infections(1%)and 8% of the strains remained untypeable.Caliciviruses.results identified: All strains belonged to NLV GVⅡ in which 6strains GⅡ-3, 2 strains GⅡ-4 and 3 stains GⅡ-7.

  Conclusion   Rotavirus is the main cause of diarrhea in chidren and caliciviruses is the next one.