Effect of vitamin E on antioxidative activity and DNA damage in rats

  Objective   To investigate the effect of vitamin E supplementation on antioxidative acivity and DNA oxidative damage in rats.

  Methods   42 weaning Wistar rats were randomly divided into 3 groups including control group, S1 and S2 groups.They were fed with fully compounded fodder for 8 weeks.The content of vitamin E in each group diet was separately 24.18, 70.83, 114.04 mg/kg.Whole blood was collected by the end of the trial.The level of plasma VE was analyzed by fluorescene spectrometry.Plasma glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)were analyzed by kits.DNA damage were analyzed by single-cell gel electrophoresis(SCGE).

  Results   The levels of plasma VE in S1 and S2 groups were higher than that of the control group.There was no difference of plasma GSH-Px and MDA between the S1, S2 groups and the control group.Intrinsic DNA damage had no differences.Induced by 5, 10 and 25μmol/L H₂O₂, DNA damage were enhanced while there were still no differences among those groups(P > 0.05).

  Conclusion   VE supplementation has no effect on the plasma GSHPx and MDA of healthy young rats and it shows no protective activity on the intrinsic and H₂O₂ induced DNA damage.