Effects of high dose VE and VC on cell activities in rats

  Objective   To study the effects of high dose VE and VC on peripheral blood lymphocyte proliferation and anti-DNA oxidative damage activities and erythrocyte membrane fluidity.

  Methods   Wistar rats were randomly divided into six groups including control, VC, VE1, VE2, VE1+VC and VE2+VC groups.The trial lasted 8 weeks.Fluorescence polarization method, methyl thiazolyl tetrazolium(MTT)and comet assay were used to detect erythrocyte membrane fluidity, lymphocyte transformation rate and DNA oxidative damage respectively.

  Results   In VE1〔33 mg/(kgºd)〕group, plasma MDA was lowered while erythrocyte membrane glutathione peruxidase(GSHPx)activity increased significantly.P(fluorescence polarization)and η(microviscosity), which were inversely related with membrane fluidity, were significantly lowered.Compared with the control, lymphocyte transformation rate in VE1 group was significantly increased by 146.54% and DNA oxidative damage induced by 10μmol/1 H₂O₂ was significantly lowered.There was significant antagonism effect between 1 000 mg/(kgºd) VC and 33 mg/(kgºd)VE and lowered cell function was seen in the combined group.

  Conclusion   Relatively high dose VE could effectively enhance lymphocyte proliferation, DNA stability and erythrocyte membrane fluidity.Buthigh dose VC could cause antagonism effect.Too excessive VE and VC showed no favourable effects and whatever more the activities of peripheral blood cell might be decreased.