Effect of vitamin E on antioxidative enzyme and ATPase activity of hepar mitochondria in rats

  Objective   To study the effect of vitamin E(VE)on antio xidative enzyme and ATPase activity of hepar mitochondria in rats.

  Methods   40 Wistar rats were randomly divided into 4 groups based on body weight, which was control, VE1, VE2, and VE3 group.The control was fed with normal rat diet and the other three inter fered groups were supplemented with different levels of VE enriched diets which contained VE 335, 1 340, 5025 mg/kg, respecively.The perido of the trial was about 10 weeks.At the end of the trial, the mitochondric were extracted from hepars.The activties Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase; Superoxide dismutase(SOD); Glutathione peroxidase(GSH-Px)and the contents of malo ndialdehyde (MDA)were measured.

  Results   Compared with the control group, VE1 group increased the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-AT Pase, SOD, GSH-Px(P < 0.05), and decr eased the contents of M DA(P < 0.05).Compared with the control group, the activities of antioxidant enzymes and ATPase in the hepar mitochondria of VE2;VE3 group had not much change.Compared with VE1 group, VE2;VE3 group decreased the activit ies of Na⁺-K⁺-AT Pase, Ca²⁺-Mg²⁺-ATPase, SOD, GSH-Px(P < 0.05), increased the contents of MDA(P < 0.05).

  Conclusion   335 mg/kg VE could increase the activities of antioxidant enzymes and ATPase.1 340, 5 025 mg/kg VE could not further incr ease the activities of antiox idant enzymes and ATPase.