Association between manganese superoxide dismutase(MnSOD)9 Ala/Val genetic polymorphisms and coronary artery disease

  Objective   To study the associations between manganese super oxide dismutase 9 Ala~Val genetic polymorphisms(MnSOD9 Ala/Val)and coronary artery disease(CAD), plasma activities of total superoxide dismutase(T-SOD), Mn-SOD, as well as plasma concentration of maleic dialdehyde(MDA).

  Methods   Using PCR-RFLP method to identify genotype of MnSOD9 Ala/Val genetic polymor phisms, and using colorimetry to detect plasma activities of T-SOD, MnSOD and plasma concentration of MDA in 262 CAD patients and 100 controls.

  Results   Compared with controls, the plasma act ivities of TSOD and MnSOD reduced obviously(23.61±16.51)U/ml vs(44.01±22.68)U/ml and(21.56±13.11)U/ml vs(28.79 ±8.65)U/ml, P < 0.001 andP < 0.001, respectively), while plasma MDAco ncentration in creased markedly(2.47± 0.73)nmol/ml vs(2.15±0.55)nmol/ml, P < 0.01).There were more AA genoty pe and A allele of MnSOD 9 Ala/Val genetic polymorphisms(64.2% vs 43.0%, P < 0.001 and 80.0% vs 67.0%, P=0.014, respectively)than controls in CAD patients.The activ ity of plasma MnSOD depressed in individuals with MnSOD AA genotype compared with those with VV genotype(22.87±13.47 vs 32.04±9.19 U/ml, P < 0.01).MnSOD correlated negatively with MDA(r=-0.15, P < 0.01).

  Conclusion   The plasma activities of T-SOD and MnSOD reduce obviously, in contrary, plasma concentration of MDA increases mar kedly in CADpat ients.The activity of plasma MnSODdepress in individuals with MnSOD AAgeno type.