Roles of heat shock protein 70 family in antigen presentation

  Objective   To study the ability o f Heat shock protein 70 family in the procession of the antigen presentation and discuss the relationship of Hsp70 family and the tumor immunogenesis.

  Methods   Antisense hsp70, ant isense hsc70 and B7 express plasmid were transfected into tumor cell line B16, and the expression of heat shock protein 70 and the effect of inhibiting Hsp on Tlymphocyte proliferation were detected after 24 hours.

  Results   The expression of heat shock protein 70 were inhibited in B16 after transfection of pLXSN-antihsp70, pLXSN-antihsc70, the efficiency of T cell proliferation was declined.In the B16 cell with pLXSNmB7, hsp70 expression were promoted and the efficiency of T cell prolifer ation was increased.

  Conclusion   Inhibiting the expression of Hsp70 family can induce the decline of the efficiency of T cell proliferation.Transfect ing pLXSNmB7 into tumor cell can improve the expression of Hsp70 family and the ability of antigen presentation.