Homology analysis of resistant plasmids in nosocomial infection Escherichia coli

  Objective   To understand the epidemic condition about the resistant plasmids and their homology of the E.coli obtained from two hospitals in Gansu province.

  Methods   The antibiotic sensitivity test, plasmid elimination, plasmidpro-file, restriction endonuclease profile were used in analyzing tho E.coli resistant plasmids.

  Results   25 strains of E.coli were isolated from 196 samples in two different hospitals, the isolation r ate was 12.8%.76.0% isolates were resistant to the eight common antibiotics in different degree.Only 28.0% strains were just resistant to one antibiotics, the multi-rcsistant strains amount for 32.0%.There were 19 isolates car ried plasmids(76.0%).The results of plasmid elimination showed 12 strains carried resistant plasmid, and the one with 24.8kb was popular plasmid.The endnuclease profiles revealed that the same source strains had certain homology, there were similiarendonuclease profiles among strains in different wards.

  Conclusion   The popular plasmids in different wards had certain homology, and they can be transferred among different strains of bacteria.