Expressions of genes related to carbohydrate and lipid metabolisms in diabetic rats with zinc supplementation

  Objective   To study the effects of zinc supplementation on related gene expression of liver in diabetic rats.

  Methods   cDNA fragments from former research project were cloned, sequenced and BLASTn analyzed.The RT-PCRs were made using the primers designed according to the sequence of cDNA.

  Results   The identities between the sequence of Zn-8 and PP2A was 99%.The expression level of DM group(0.5072±0.0574)was obviously lower than those of normal group(1.3303±0.1855, P < 0.05).And The expression level of DM+Zn group(0.7005±0.1563)had some improvement compared with those of DM group(P < 0.05).

  Conclusion   Zinc supplementation can improve the expression of PP2A mRNA on liver in diabetic rats.It may be one of the molecular mechanisms that zinc can improve the disorders of glucose and lipids metabolism in diabetic rats.