Relationship between expression of HBVx-DNA and mIL-2R in peripheral blood of the patients with chronic hepatitis B

  Objective   To study the relationship between the expression of HBVx-DNA in serum and the level of membrane interleukin-2 receptor(mIL-2R)on surface of peripheral blood mononuclear cells(PBMC)of the patients with chronic hepatitis B and pr imary hepatoma.

  Methods   The HBVx-DNA in serum and the level of mIL-2R before and after inducement of PHA were detected by PCR and biotin-streptav idin(BSA), respectively.

  Results   The total positive rates of HBVx-DNA in serum of the patients with chronic hepatitis B and primary hepatoma were 11.9%(16/135), 40.9%(9/22), respectively.There was high significantly difference in two groups(P < 0.01).The positive rates of HBVx-DNA in serum of the patients with chronic hepatitis(light), chronic hepatitis(moderate), chronic hepatitis(severe), cirrhosis were 0%, 20.8%(5/24), 17.9%(5/28), 17.1%(6/35), respectively.There was high significantly difference among these groups (P < 0.01).The total positive rates of HBVx-DNA in serum of group with HBeAg(+)was 2014%(21/103).There was higher significantly difference in group with HBeAg(+)than that in group with HBeAg(-)(P < 0.05).The levels of mIL -2R before and after inducement of PHA on surface of PBMC of group with HBV x-DNA(+)and HBVx-DNA(-) were(2.53±1.08)%, (26.47±4.26)%and(3.34±1.23)%, (29.25±4.71)%, respectively.There was higher significantly difference in the two groups(P < 0.01).The results of liver biopsy showed that the highest level of HBVx-DNA in tissue of hepatoma.

  Conclusion   HBVx-DNA can be existent in the patients with chronic hepatitis B and is more common in chronic hepatitis B(moderate, severe)and cirrhosis.The positive rate of HBVx-DNA in serum of the patients with primary hepatoma was 50%(3/6)and was hig her than that of patients with chronic hepatitis B(moderate, severe)and cirr hosis.The main reason of primary hepatoma is associated with HBVx-DNA integr ated with chromosome of host cells.The other important reason is that the lowactive T cells is more common in peripheral blood of the patients with HBVx-DNA(+).It is high significant to detect the level of HBVx-DNA in serum and PBMC forearly discovery and assistant diagnosis of primary hepatoma.