Effect of hematite nanoparticles on membrane fluidity and experiments on genotoxicology

  Objective   To investigate the oxidative injur y of hematite nanoparticles from the point of influence on membrane fluidity and its genotoxicolog y effects in vitro.

  Methods   To determinate the fluorescent intensity by spectroflurophoto meter with 1, 6-dipheny-l 1, 3, 5-hex atriene(DPH)as a fluorescein mark, thus to calculate the value of fluidity degree according to the specific for mula with fluor escent intensity data.To observe the damage of hematite nanopar ticles to DNA by single-cell gel electrophoresis(SCGE)test in vitro.

  Results   Decrease of membrane fluidity was found in group of dose 128μg/ml, however no obvious damage to DNA obser ved at every dose level of 8, 32, 128 μg/ml.

  Conclusion   Under the condition of our test, it is demo nstrated from the experiment results in vitro that Fe ₂O₃ nano particles may induce the oxidative stress in cells and consequently lead to lipid peroxidation on membr ane, while no obvious damage to genetic materials in cells.