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GUO Xiao-peng, HUANG Guo-xiang, WU Qing, . Effect of manganese on growth of HUVEC-304 cells and expression of caspase[J]. Chinese Journal of Public Health, 2007, 23(1): 110-111. DOI: 10.11847/zgggws2007-23-01-64
Citation: GUO Xiao-peng, HUANG Guo-xiang, WU Qing, . Effect of manganese on growth of HUVEC-304 cells and expression of caspase[J]. Chinese Journal of Public Health, 2007, 23(1): 110-111. DOI: 10.11847/zgggws2007-23-01-64

Effect of manganese on growth of HUVEC-304 cells and expression of caspase

  • ObjectiveTo investigate the effect of manganese on growth of HUVEC-304 cells and the ex pression of caspase-8、caspase-3.MethodHUVEC 304 cells were given with various concentrations of MnCl2.The inhibitory ratio was measured by MTT assay.Apoptosis was observed by flow cytometry(FCM).The expression of caspase-3 and caspase-8 was measured by Western blot.ResultsMTT results revealed that manganese chloride(from 100 to 800 μmol/L)might suppress the proliferation of HUVEC-304 cells(inhibitory ratio were 22.34%~90.94%)and induce apoptosis(apoptosis ratio were 10.20%~98.73%)in dose and time-dependent manner.The result of Western Blot showed that manganese inhibited caspase-3 and caspase-8 expression(P<0.05).ConclusionManganese can inhibit proliferation of HUVEC-304 cells with time-and dose-dependent manner.These may be one of important mechanisms that manganese suppressed the proliferation of HUVEC-304 cells and induced apoptosis.
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