Study on p53, rasp21 and p16 expression in NIH/3T3 cells treated by volatile organic compounds

ObjectiveTo study the expression of p 53、rasp 21、p 16 in NIH/3T3 cell treated by VOCs(the compounds of benzene and formaldehyde).MethodsUsing SABC methods of immunohistochemistry to observe the expression of p 53、rasp 21、p 16 in NIH/3T3 cell.ResultsThe expression of p 53 and rasp 21 increased in VOCs groups compared with negative control group(P<0.01),but the deletion of p 16 increased compared with negative control group(P<0.01).The positive rate of p 53 and rasp 21 were 26%~45% and 50%~60%,respectively.The positive rate of p 16 was 64%~40%.ConclusionOver expression of p 53,p21 and deletion of p 16 maybe one of the major reason in the occurrence of neoplasm caused by VOCs.