Changes of certain biochemical indexes of testis in rats exposed to hypoxia

ObjectiveTo explore the effects of hypoxia on the environment of spermatogenesis in rat.MethodsAdult male Wistar rats were randomly divided into four groups:control group raised at sea level;5,15 and 30 d hypoxic group raised in a hypobaric chamber stimulating 5 000 m altitude for 5,15 and 30 days respectively.Lactate dehydrogenase C4/lactate dehydrogenase(LDH-C4/LDH),gamma-glutamyl transferase(GGT),fructose and malondialdehyde(MDA)were measured in testis.ResultsLDH-C4/LDH in hypoxic 30 d group was significantly lower than that in control group(P<0.05).Activity of GGT in hypoxic 30 d group was significantly higher than that in control group(P<0.05).Fructose level in testis was not significantly changed compared with control group.MDA decreased significantly when exposed to hypoxia (P<0.05).ConclusionHypoxia changes the environment of spermatogenesis in rat.