Effects of manganese on glutamate metabolism and enzyme activities in brain of rats

ObjectiveTo study the effects of different doses of manganese on activities of GS and PAG in the rat striatum and the activities of SDH and Na⁺-K⁺-AT Pase in pallium in order to explore the cell molecular mechanism of man ganese-induced exeitotoxicity.MethodsThe Wistar rats were divided into four groups by weight at random.The first group was the control group which was given peritoneal injection(i.p)of 0.9% NaCl;the second to fourth groups were respectively given i.p of 8,40,200 μmol/kg MnCl₂.The capacity of injection was all 5 ml/kg.After 25-days administration, the activities of GS and PAG in the rat striatum and the activities of SDH and Na⁺-K⁺-AT Pase in pallium were determined.ResultsAccompanied with the incrasing of treated Mn dosage,the activities of SDH and Na⁺-K⁺-AT Pase in pallium decreased,the activities of SDH and Na⁺-K⁺-AT Pase in pallium in 200 mol/kg MnCl₂ group decreased significantly with a dose-response relationship;the activity of PAG in corpor a striatum increased and the activity of GS in corpora striatum decreased in 200 mol/kg MnCl₂ group;the activity of GS in corpor a striatum decreased significantly,however,the activity of PAG in corpora striatum increased significantly.ConclusionMn may decrease the activities of SDH and Na⁺-K⁺-ATPase in pallium and the activity of GS in corpora striatum,and increase the activity of PAG in corpora striatum,which led to disrupt glutamate metabolism.