Inhibitory effect of plant tannins on butyric acid and hemin-induced erythroid differentiation of K562 cells

ObjectiveEffects of plant tannins,chebulinic acid and tellimagrandin I on chemically induced hemoglobin synthesis in K 562 cells were investig ated.MethodsThe hemoglobin synthesis situation was assayed with benzidine staining.Erythroid antigens glycophorin A(GPA)expression on the surface of K 562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-GPA antibodies.Then flow cytometric analysis was performed to detect GPA expression levels on surface of the cells.ResultsBoth chebulinic acid and tellimagrandin Icould inhibit the hemoglobin synthesis of butyric acid and hemin-treated K562 cells in a concentration-dependant manner.However,the BA-induced K562 cellscere more sensitive to two tannins than heroin-induced cells.ConclusionChebulinic acid and tellimagr andin I have inhibitory effect on the erythroid differentiation.