Effect of low intensity pulse ultrasound on proliferation of human skin fibroblasts

Objective To determine whether low intensity pulse ultrasound has any effect on cell proliferation of human skin fibroblasts in vitro.Methods The proliferation effects of human skin fibroblasts were determined by BrdU incorporation assays.Results The proliferation of human fibroblasts was deter mined after 5,8,11,and 14 minutes of low intensity pulse ultrasound treatment.In cell culture without FCS DM EM medium,there was a significant increase of stained cell number after 8 and 14 minutes of treatment(P<0.05 and P<0.01,respectively).In the cell culture with 10% FCSDMEM medium,there was a significant increase of stained cell number after 5,8,11 and 14 minute treatment(P<0.01).Conclusion Low intensity pulse ultrasound has stimulation effect on cell proliferauon of human skin fibroblasts in vitro.In addition,the proliferation effect can be affected by concentration of FCS in the culture medium and the treatment time.