Effect of acrylamide on proliferation and differentiation of rat kidney epithelial cells in vitro

Objective To study the effect of acrylamide on proliferation and differentiation of rat kidney epithelial cells.Methods The primary culture of kidney epithelial cells of new born rat were separated,and digested after purification.Acrylamide was added at the dose of 0.1,1.0,10.0,50.0,100.0 μg/ml,respectively.The cell culture was observed with cytomorghology and cell counting.Concentration and the content of protein,the MTT,malo ndialdehyde(MDA)and activity of superoxide dismutase(SOD)were determined.Results Acrylamide inhibited differerentiation of the cells by affecting cell proliferation.The inhibition rate was 97%,86%,80%,56% and 38%,respectively.The content of protein was 92%,90%,85%,53% and 33% of that control group,respectively.The results showed that acrylamide(10.0,50.0,100.0 μg/ml) could inhibit differentiation of kidney epithelial cells without affecting cells proliferation.The level of MDA in groups treated with acrylamide showed increasing tendency.The activity of SOD was lower than that of control group,with a significant decreasing in the 10.0 μg/ml group,50.0 μg/ml group and 100.0 μg/ml group.Conclusion The inhibition of acrylamide on rat kidney epithelial cells might be associated with the impact of protein and DNA synthetis.