Reverse effects of α-NF pre-treatment on protein levels of P53, P21 in HELF cells induced by B(a)P

ObjectiveTo investigate the effects and mechanisms of B(a)Pon P53,P21 and AhR pathway of HELF cells in vitro.MethodsDifferent doses of α-NF,AhR an tagonist were used to pre-treat human diploid lung fibroblast (HELF)cells for 1h,followed by B(a)Ptreatment for additional 2h with or without S₉,or followed by B(a)P treatment for additional 24h without S₉.The prote in levels of P53,P21 were detected by western blot to investiga te AhR pathway's role involving in the effects of B(a)P on P53,P21.ResultsPre-treatment with α-NF at doses of 2.5 mol/L or 5.0 mol/L could reverse the increase of P53 levels with B(a)P treatment for 2h without or with S₉,respectively.Pre-treatment with α-NF at doses of 2.5 mol/L and 5.0 mol/L could reverse the increase of P53 levels with B(a)Ptreatment for 24h without S₉.Pre-treatment with α-NF at doses of 2.5 mol/L and 5.0 mol/L could reverse the increase of P21 levels with B (a)Ptreatment for 24h without S₉.ConclusionP53,P21 play important roles in the response of HELF to DNA damage in duced by B(a)P,in which AhR pathway takes part in there sponse.