Effects of curcumin on proliferation of hepatic satellite cell in vitro

Objective To investigate the effects of curcumin on proliferation and collagen(col)secretion of hepatic satellite cells in vitro.Methods CSFSC-2G were incubated with different concentration of curcumin(0,5,10,15,20,40 μmol/L)for different times(6h,12h,24h,48h and 72h).MTT was used for the detecting of proliferation of HSC.Col Ⅰ and Col Ⅲ were determined by ELISA and flowcytometry was applied to evaluate apoptosis of HSC.Results Curcumin inhibited the proliferation of HSC with dose-dependent manner,especially in 20 and 40 μmol/L curcumin groups at 24h.When HSC was cultured after 24h,the absorbency of Col Ⅰ and Col Ⅲ in 20 μmol/L curcumin group was 0.418±0.019 and 0.554±0.043,respectirely,both lower than those of 0 μmol/L curcumin group significantly(P<0.01).When HSC was cultured after 48h,the ratio of apoptosis in 20 μmol/L curcumin group was 20.31%±2.55% and higer than that of 0 μmol/L curcumin group significantly(P<0.01).Conclusion Curcumin can delay liver fibrosis not only by inhibiting the proliferation and stimulating apoptosis of HSC,but also by decreasing Col Ⅰ and Col Ⅲ secretion.