Comparison of curcumin-induced damage of mitochondrial DNA and nuclear DNA by long PCR

Objective To compare the damage effects of crown in on mitochondrialDNA (mtDNA)and nuclea DNA (nDNA)in human colon cancer cell line HT-29.Methods After HT-29 cells were exposed to various concentrations of cureum in (0,2.5,5.0,10.0,20.0μg/ml) for 2h,high molecular weight DNA was isolated and long polymerase chain reaction (LPCR) assay was perfouned to deteun ine the damage of mtDNA and nDNA.Results A 2h exposure of HT-29 cells to cureum in led to a dose-dependent decrease in the amplification of both mtDNA and nDNA.Loss of amplification of the mtDNA occurred at a lower dose of curcum in (5μg/ml) compared with the nDNA(10μg/ml).For mtDNA,DNA lesion frequencies for groups with different concentrations of cureum in (5,10 and 201μ/ml) were 1.23±0.10,1.97±0.24 and 5.78±0.62 lesions/10kb(P<0.01),and that of for nDNA were 0.27±0.04,0.61±0.11 and 1.61±0.12 lesions/10kb,respectively.Conclusion In HT-29 cells,curcum in induces DNA dam age to both m1DNA and nDNA,and the damage to mtDNA is more serious as compared with nDNA.