HSP70 subtypes expression in human embryonic lung fibroblasts induced by supernatants of alveolar macrophage exposed to silica

Objective To observe the expression of HSP70-1,H SP70-2,HSP70-hom in human embryonic lung fibroblasts(HELF)induced by supernatants of alveolarm acrophage(AMs)exposed to silica.Methods AMs and HELF of rats were formed invitro model.The HSP70-1,HSP70-2,HSP70-hom mRNA levels of H ELF stmiulated by supernatants of AMs exposed to silica were detected by RT-PCR technology.Results Expression of HSP70-1,HSP70-2,HSP70-hom could be induced by dust-exposed AMs and there was a clear dose-effectrelationship between HSP70-1,HSP70-2,HSP70-hom mRNA levels and silica concentration with the correlation coefficients of 0 .931(P<0. 01),0. 923(P<0 .01),0. 818 (P<0.05),respectively.Conclusion HSP70-1,HSP70-2,HSP70-hom may play an antioxidant role in the process of oxidative stress induced by silica.