Subtype distribution of HIV-1 among different people infected

Objective To investigate the subtypes and distribution characteristics of HIV1 among different populations infected.Methods HIV1 proviral DNA was extracted from the whole blood of 148 HIV1 infected indi iduals.The HIV-1env C2-V3 gene region was amplified by nested PCR and was sequenced.The sequence was compared with HIV-1 subtype reference sequence.Results There were 8 kinds of HIV-1 subtypes in 148 samples.In the subjects,45 case swere Bsubtype;37 were CRF07 BC;35 were B';25 were CRF01-AE;2 cases were C and CRF02-AG;1case was CRF03-AB and CRF02-AG.Conclusion HIV-1 subtypes in the blood donors and blood recipients and in drug users were not very different.Sexually transmitted HIV-1 subtype was distributed widely.