Application of real-time PCR for detection of Neisseria meningitides

Objective To evaluate the application value of a new type probe(TaqMan MGBprobe)in real time PCR for detection of Neisseria meningitides.Methods Overall 230 samples of throatswabs from healthy people were detected with the established TaqMan MGB realtmie PCR,simple PCR and conventional methods.The detection rates of the three methods were compared to analyze their differences.Results The positive rate of Neisseria meningitides detected by Taq Man MGB probereal time PCR(10.47%)was significantly higher than conventional methods(3.48%)and smiple PCR(6.25%,P<0.001).Conclusion The TaqMan MGB probe real time PCR is a rapid,high sensitive and specific method in the detection of Neisseria meningitides,which could provide a rapid and convenient etiological diagnosis method for Ne is seria meningitides in epidemiological investigation.