Differential protein expression between oral squanmous carcinoma parental cells and multidrug resistance cells in vitro

Objective To investigate differential protein expressions between oral squamous carcinom a cell line KB and multidrug resistance cell line KBV 200 in vitro.Methods The surface enhanced laser desorption ionization(SELDI)was used.The KB and KBV 200 cells were harvested and then split by cell ly sate solution.The prote in mass spectra of the KB and KBV 200 cells was detected by CM 10 chip based on SELDI prote in chiptechnology.Results A total of 102 prote in swere captured by CM 10 chip,and 19 differential peaks were found.Among those differentially expressed proteins,15 were highly expressed,while 5 were lowly expressed in KBV 200 cells.Conclusion SELDI technology is convenient,sensitive,and repeatable in the detection of the different proteins expressions between oral squamous carcinom a cell line KB and multidrug resistance cell line KBV 200.