Inhibitive effect of hgdroxytyrosol on Sudan Ⅰ induced DNA oxidative damage in human liver-derived HepG2 cells

Objective To explore the inhibitory effect of hydroxy tyrosol(HT)on oxidative injury induced by Sudan Ⅰin HepG2 cells.Methods The single cell gel electrophoresis assay(SCGE)was performed to study the DNA damage.The in tracellular reactive oxygen species(ROS)formation was measu redusing 2,7-dichloro fluorescein diacetate(DCFH 2DA)as a fluorescent probe.The levels of oxida tive DNA damage and lipid peroxidation were estimated by immunocy to chemistry analysis of 8-hydrocyde-oxyguanosine(8-OHdG)and by measuring the level of thtiobarbituric acid-reactive sub-stances(TBARS).Results Com pared with the control group,HepG2 cells treated with 100μMSudanⅠshowed significantly different DNA strand breaks(tail length of com et:46.49±2.06μm,the ta ilmoment:19.99±1.44μm),and the increases of ROS(9.03±1.05μm measured by fluorescent in tensity),in tracallular TBARS level(0.41±0.15μm)m easured by absorbency,and the increase of 8-OHdG expression(20.5±0.09μm measured by rleative staining intensity).Pretreatment with HT could inhibit the DNA damage and decrease the level of ROS,TBARS and 8-OHdG in a concentration-dependent manner.Conclusion HT could modulate the redox state and prevent the oxidative damage induced by SudanⅠin HepG2 cells.