Anti-proliferative effect of δ-tocotrienol on colorectal cancer cells

Objective To investigate inhibitory effect of δ-tocotrienol on prolife ration of colorectal cancer(CRC) SW 620 cells.Methods SW620 cells we retreated with different concentration of δ-tocotrienol(0,20,30,40,60 mol/L).Methyl thiazolylte trazolium(MTT) method was adopted for the detection of cell growth and real time reverse transcription polymerase cha in reaction(RT PCR) for the detection of corre lated mRNA expression.The contents of GSK-3β,APC,and Ax in mRNA were detected.Results δ-tocotrienol inhibited proliferation of CRC-SW 620 cells.After treated with δ-tocotrienol for 24 hr,the survival rate of the cells was decreased by 14.4% to 88.8%.The RT PCR study showed that δ-tocotrienol promoted the expression of Ax in mRNA(F=6.598,P < 0.05),but not the GSK-3β mRNA and APC mRNA(P >0.05).Conclusion δ-tocotrienol can inhibit SW 620 cells proliferation.Its inhibitory mechanism correlates possibly with the adjusting of the associated expression of Ax in mRN A.