Cloning and immunoreactivity of full-length cDNA sequence of Taenia saginata asiatica eukaryotic translation initiation factor 3 subunit

Objective To clone and express the gene named as eukaryotic translation initiation factor 3 subunit(EIFs3)of Taenia saginata a sitica and to analyze the mimunogenicity of its recombinant protein.Methods By online analysis of the gene at bioinfom aticswebsites,the gene from the Taenia saginata a siatica full length cDNA plasmid libratory was identi fied and its coding region sequence was analyzed.Then the coding region of the gene was amplified with PCR.The recombinant prote in was detected by sodium dodecyl sulfate polyacry lamide gelelec trophoresis(SDS PAGE)after being induced with isopro pyl thiogalacto side(IPTG)in Ecoli BL 21/DE3 and purified with NI IDA affinity chroma to graphy.In addition,the immuno reactivity of the purified recombinant prote ins was analyzed with We stern blotting.Results As demonstrated by PCR,double enzyme digestion and DNA sequencing of the recom binant plasmid was successively constructed.SDS PAGE results showed that the gene was expressed in E coli BL 21/DE3 and the recom binant prote in could react with Taenia a siatica andtae niarhynchus saginatus infected patientse rum,which indicated its mimuno reacticity.Conclusion The EIFs 3 of Taeniasaginata a sitica was cloned and expressed,and the purified protein has mimunogeni city.