Effect of combined polysaccharides on radiation-induced damage of testis tissue in rats

ObjectiveTo study the combined effect of the Laminaria japonica polysaccharides(LJP)and Lycium barbarum polysaccharides(LBP)on male rats'testicular tissue oxidative damage induced by chronic local ionizing radiation.MethodsFour weeks old male Wistar rats were randomly divided into the control,model,LBP,LJP,and LBP+LJP group.After one week adaptable rearing,apart from the control group,all groups were exposed to ⁶⁰Coγ-ray local irradiation everyday for 20 days in four weeks(except the weekend)at a total radiation dose of 2.3 Gy for each rat.During the radiation,the rats were given intragastral administration according to the grouping everyday.Seven days after the cessation of exposure,the testicular tissue was observed and the levels of superoxide dismutase(SOD),glutathione(GSH),glutathione peroxidase(GSH-PX)and content of malonodialdehyde(MDA)in testicular tissue were determined.ResultsTesticular tissue in LBP+LJP was normal.LBP+LJP increased the contents of SOD,GSH-Px,GSH(P<0.01)and reduced MDA content(P<0.01).ConclusionThe radioprotective effects of combined LBP and LJP on testicular tissue are obvious and the combined effect is better than LBP or LJP independently.