Isolation of Ochrobactrum anthropi with modified cefoperazone Mac-Lonkey agar

ObjectiveTo know the morphology,culture,biochemistry,and drug sensitivity of Ochrobactrum anthropi and to establish a suitable methods for its identification.MethodsOchrobactrum anthropi was isolated with modified MacLonkey agar(with cefoperazone added)and identified by API20E,VITEC2GN.The drug sensitivity of Ochrobactrum anthropi was determined with AST-GN10.ResultsFrom 206 samples,there were 21 Ochrobactrum anthropi strains isolated.The isolated strains could not utilize glucose and alcohol.The results of drug sensitivity test on Ochrobactrum anthropi strains showed minimal inhibitory concentrations(MIC)of ≥128 for ticacilin and piperacilin,≥64 for cephalosporin antibiotics,and≤1 for tetracycline,levofloxacin,ciprofloxacin,meropenem,and imipenem.ConclusionModified Mac-lonkey agar(with cefoperazone added)is suitable for the detection and identification of Ochrobactrum anthropi in clinical laboratory and natural environment.Ochrobactrum anthropi is highly resistant to β-lactamase-producing antibiotics.