Proteomic analysis of differential proteins in interaction of Helicobacter pylori and U937 cell line

ObjectiveTo search for differential proteins after the interaction of Helicobacter pylori(Hp)and host cells (U937).MethodsTwo Hp strains(26695 and TN2)and cell line U937 were used.Two-dimensional electrophoresis (2-DE)and mass spectroscopy were applicated to separate protein and to identify differential protein.The protein maps were then compared by ImageMaster 2Dv3.1.ResultsTwo differential protein spots were found in the gel of 2-DE and the one was identified as heat shock protein 60(Hsp60).ConclusionHp Hsp60 could enter the host cell or connect to the membrane of the host cell and the mechanism needs to be explored.