Genotype analysis of varicella-zoster virus in Liaoning province

ObjectiveTo examine the genetype of varicella-zoster virus in Liaoning province.MethodsThe serum and throat swabs speciments were collected from 7 patients clinically diagnosed with varicella for the detection varricellazoster virus(VZV)IgM and the amplification of DNA of the open reading frame(ORF)22.The DNA was sequenced and analyzed with the data of VZV reference strains downloaded from GenBank.ResultsAll the 7 patients were VZV IgM positive and the amplification,and sequencing of 3 throat swabs(VZV001,VZV-002,VZV-005)confirmed the VZV.The results of ORF22 single nucletotide polymorphism(SNP)analyses showed that VZV-002 and VZV-005 had the same sequence and the single base transvertion was A→C.The sequence of VZV-001 had 2 changed bases,suggesting the point mutation of the gene.compared with Dumas strain,the 3 strains had 4 bases different on 6 key site and completely matched with P-Oka strain.The 3 strains were identified as J genotype and the nucleotide homology was 99.56%-99.78% with P-Oka.ConclusionThe genotype of 3 VZV strains detected in Liaoning province belongs to J genetype.