Effects of secreting type antibacterial peptides from Musca domestica larvae on immune function in mice

Objective To study the influence of secreting type antibacterial peptides from Musca domestica larvae on the immune system of normal mice.Methods Thirty-two BALB/c mice were selected and divided randomly into four groups.Different doses of secreting type antibacterial peptides(5,10,20 mg/kg)were administered to the mice with intraperitoneal injection for 7 days.A series of classical tests were adopted to evaluate the effects.Results In the 10 mg/kg and 20mg/kg dose groups,the ratio of spleen/body weight were 7.41±0.93 and 7.56±1.05;the phagocytotic ability of macrophages were 38.81±7.88 and 40.16±9.74;the lymphocyte transformation induced by conA of the mice were 0.31±0.11 and 0.32±0.09.All the indexes were higher than those of the control with statistically significant differences(P<0.05 for all).But the body weight and the 50 percent hemolyzingdose of complement of the 5-20mg/kg dose groups were not different from that of the control group.Conclusion The secreting type antibacterial peptides from Musca domestica larvae could promote the immune function in normal mice.