Clonging and sequencing of sugar fermentation stimulation protein gene from Vibrio cholerae 04-5a

Objective To investigate the differences in the sequences of sugar fermentation stimulation protein genes between Vibro cholerae 04-5a and epidemic and nonepidemic V.cholerae strains.Methods Cloning and sequencing methods were used to analyze the sequence of sugar fermentation stimulation protein genes from V.cholerae 04-5a and to compare the sequences with related gene sequences.Results The sequence of sugar fermentation stimulation protein genes from V.cholerae 04-5a is a 771bp gene,encoding 258 amino acid polypeptide,and is identical to the ones from the epidemic strain.According to the sequence of sugar fermentation stimulation protein gene,the V.cholerae 04-5a has mutations at 334th,622th and 666th point compared with the nonepidemic strain,and is identical to the epidemic strain.The difference of the 666th point mutation led to the changs of the deduced amino acid level.Conclusion The sequence of sugar fermentation stimulation protein gene from V.cholerae 04-5a is identical to that of from the epidemic strains and the related pathogenic mechanism and epidemiology need to be further studied.