Relationship between calciotropic hormone levels and gene polymorphisms of estrogen receptor and vitamin D receptor among children

Objective To explore the relationship between calciotropic hormone levels and gene polymorphisms of estrogen receptor(ER)and vitamin D receptor among children.Methods Totally 140 healthy Chinese children aged 8-12 years living in Henan province were recruited.Polymerase chain reaction-restricted fragment length polymorphisms(PCR-RFLP)method was used to detect PvuⅡ and XbaⅠ polymorphisms of ERα gene and FokⅠ polymorphism of vitamin D receptor(VDR)gene.Calcitonin(CT)and osteocalcin(OC)levels in serum were measured using radioimmunoassay.Results Serum OC concentration of the children with different genotypes of ER PvuⅡ were 5.82 μg/L for PP,5.01 μg/L for Pp,and 6.21 μg/L for pp.Serum OC concentration of the children with pp genotype was significantly higher than those with PP and Pp genotypes(P < 0.05).However,the differences between serum Ca and serum CT were not observed in different ER PvuⅡ genotypes(P > 0.05 for all).There were no significant differences in serum CT and OC concentrations among children carrying different FokⅠ genotypes of VDR gene(P > 0.05).Serum Ca concentration of the children with different genotypes of VDR FokⅠwere 2.71 mmol/L for ff,2.39 mmol/L for Ff,and 2.48 mmol/L for FF.Serum Ca concentration in the children carrying ff genotype was significantly higher than that of the children carrying FF and Ff genotypes(P < 0.05).Conclusion This study provides the evidence of an association of ER PvuⅡ and VDR FokⅠpolymorphisms with osteocalcin and Ca in serum of children.