Lead acetate-induced cytotoxicity in Z310 cells

Objective To detect hormesis effects of lead acetate on Z310 cells.Methods Z310 cells were treated with lead acetate at concentrations of 0,0.000 2,0.002,0.02,0.2,2,20,200 and 500 μmol/L for 12 hours and 24 hours.The proliferation viability of Z310 cells was measured by 3-(4,5-dimethythiazol-2-yl) -2,5-diphenyltetrazolium bromide(MTT) and 2-(2-methoxy-4-nitrophenyl) -3-(4-nitrophenyl) -5 (2,4-disulfophenyl)-2H-tetrazolium,monosodium salt (WST-8) assay.And morphological changes in Z310 cells were observed under optical microscope.Results Lead acetate stimulated cell survival rate (107.06%) at lower concentration(0.02 μmol/L) for 24 hours.Compared with the control,a significant survival rate increase(110.91%,P< 0.05) was observed following exposure to 0.2 μmol/L lead acetate for 24 hours,but at higher concentrations(over 2 μmol/L),the survival of the cells was inhibited.The survival rates significantly decreased(79.37% and 76.81%) only at 200 and 500 μmol/L lead acetate for 24 hours tested by MTT(P< 0.01).The same effects were observed by WST-8 at the same concentration,with the survival rates of 81.67% and 72.36% for 12 hours(P< 0.01) and 56.89% and 44.05% for 24 hours(P< 0.01).Conclusion Lead acetate can induce the hormesis of Z310 cell proliferation.There is a higher sensitivity in cell survival rate test with WST-8 than MTT.