Function of lipid rafts in CVB3 infection on cardiocytes

Objective To explore the role of lipid rafts in coxsackievirus B3(CVB3)-infected cardiocytes.Methods Methyl-β-cyclodextrin(MβCD) was used to remove the cholesterol molecules maitaining the stability of lipid rafts on cell membrane,then the cells were infected with CVB3;western blot was adopted to detect the expression of CVB3 VP1 protein.Virus titer was determined after adding exogenous choleserol to observe inhibitive effect of MβCD on CVB3 infection.Results The damage of cell membrane lipid rafts duo to the removement of the cholesterol molecules by MβCD could inhibit the infection of CVB3 to cells and reduce the virus titer,and exogenous cholesterol could regain the inhibition of MβCD to CVB3 infection.Conclusion The lipid rafts on cardiocytes membrane plays an important role in CVB3 infection to the host cell.