PFGE type and serotype of Salmonella strains

Objective To analysze serotype and pulsed-field gel electrophoresis(PFGE) type of Salmonella strains isolated from food samples collected in Jilin province and to explore molecular polymorphism of Salmonella.Methods Totally 28 Salmonella strains were isolated from raw chicken meat and Chinese salad sampled in markets and genotyped with serologic method and PFGE.The homology of the strains was analyzed with BioNumerics 6.0.Results The serotypes of the 28 Salmonella strains were 15 Salmonella enteritidis,4 Salmonella indiana,2 Salmonella Cholerae-Suis,2 Arizona,and 5 undetermined serotype.Based on PFGE results,the 28 strains were clasified into 4 major types,including 14 strains of type 1 with the similarity of more than 87.3%,7 strains of type 2(6 strains of 2A subtype with the similarity of more than 91.9%,one strain of 2B subtype with the similarity of more than 73.6%).Conclusion The main serotype of Salmonella isolated from food samples in Jilin province is Salmonella enteritidis.PFGE results show that the Salmonella strains of the same serotype are highly homologous.