Expressions of PPAR and UCP-2 mRNA in hepatic tissue in NAFLD mice

ObjectiveTo explore the expressions of peroxisome proliferator-activated receptor α(PPARα)and uncoupling proteins 2(UCP-2)in hepatic tissue in the mice with non-alcohlic fatty liver disease(NAFLD).MethodsThirty mature male ICR mice were randomly divided into three groups(10 in each group).The mice in control group were fed with normal diet;the mice in experimental group A were fed with normal diet for 4 weeks;and then with high-fat diet for 4 weeks;and the mice in experimental group B were fed with high-fat diet for 8 weeks.Liver index were measured.The expressions of PPARα and UCP-2 mRNA in hepatic tissue were examined with reverse transcription polymerase chain reaction(RT-PCR).ResultsThe liver index for the mice in control group,experimental group A and experimental group B were 3.89±0.87,7.42±0.95,and 9.38±1.07,respectively.In the mice of experimental group,the liver index was significantly higher than that of the control mice(P<0.01).The steatosis of hepatic tissue in experimental group was obvious.PPARα mRNA expressions in hepatic tissue of mice of experimental group A(0.63±0.33)and experimental group B(0.45±0.19)were dramatically decreased compared to normal group(1.16±0.27)(P<0.01).UCP-2 mRNA expressions in hepatic tissue of mice of experimental group A(0.67±0.76)and experimental group B(0.89±0.52)were dramatically increased compared to normal group(0.25±0.13)(P<0.01).ConclusionThe expression of PPAR α and UCP-2 in hepatic tissue of NAFLD mice are abnormal.