Genotyping of hepatitis B virus among Han and Mongolian populations in Keyouzhong banner of Inner Mongolia

ObjectiveTo investigate the distribution of hepatitis B virus(HBV) genotype among Han and Mongolian in Keyouzhong banner of Inner Mongolia.MethodsWe used sequencing and phylogenetic analysis to determine the distribution of genotypes,subtypes and S mutations of HBV.HBV serologic markers were detected with enzyme-linked immunosorbent assay(ELISA).ResultsFor 105 Mongolian,93 were sequenced successfully,with 26 genotype B2(27.96%),47 genotype C2(50.53%),and 20 genotype D1(21.51%).For 60 samples from Han,51 were sequenced successfully,with 10 genotype B2(19.61%),32 genotype C2(62.74%),and 9 genotype D1(17.65%).All genotyping results based on Basic Local Aligment Search Tool(BLAST) of National Center for Biotechnology Information(NCBI) were identical to those based on phylogenetic tree.ConclusionThe genotypes of HBV are genotype B,C and D among Han and Mongolia populations in Keyouzhong banner of Inner Mongolia,with dominant genotypes of band D in Mongolia and C in Han population.